Ultrahigh accuracy imaging modality for super-localization microscopy
نویسندگان
چکیده
منابع مشابه
Super-Resolution Localization Microscopy
Light microscopy techniques have been vital to our understanding of biological structures and systems since their invention in the late 16 Century. However, conventional light microscopy techniques are limited by the diffraction limit of light, preventing us from seeing anything smaller than approximately 200 nm in x and y and approximately 500 nm in z. Many molecules and structures of interest...
متن کاملTranslation Microscopy (TRAM) for super-resolution imaging
Super-resolution microscopy is transforming our understanding of biology but accessibility is limited by its technical complexity, high costs and the requirement for bespoke sample preparation. We present a novel, simple and multi-color super-resolution microscopy technique, called translation microscopy (TRAM), in which a super-resolution image is restored from multiple diffraction-limited res...
متن کاملComputational Image Analysis Techniques for Super-resolution Localization Microscopy Computational Image Analysis Techniques for Super-resolution Localization Microscopy
Super-resolution microscopy techniques, which overcome the diffraction limit of optical microscopy, are revolutionizing biological research by providing the ability to localize proteins within cells at nanometer resolution. Super-resolution localization microscopy (SRLM) is one of such techniques. It achieves nanometer resolution by randomly activating separate fluorophores and computationally ...
متن کاملSuper-resolution spectroscopic microscopy via photon localization
Traditional photon localization microscopy analyses only the spatial distributions of photons emitted by individual molecules to reconstruct super-resolution optical images. Unfortunately, however, the highly valuable spectroscopic information from these photons have been overlooked. Here we report a spectroscopic photon localization microscopy that is capable of capturing the inherent spectros...
متن کاملLocalization-Based Super-Resolution Light Microscopy
Kristin A. Gabor,1,2,3 Mudalige S. Gunewardene,1 David Santucci,4 and Samuel T. Hess1,3,* 1 Department of Physics and Astronomy, 120 Bennett Hall, University of Maine, Orono, ME 04469 2 Department of Molecular and Biomedical Sciences, 5735 Hitchner Hall, University of Maine, Orono, ME 04469 3 Graduate School of Biomedical Sciences, 263 ESRB/Barrows Hall, University of Maine, Orono, ME 04469 4 V...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Nature Methods
سال: 2013
ISSN: 1548-7091,1548-7105
DOI: 10.1038/nmeth.2396